Single-mode tapered optical fiber immunosensor I: characterization with model analytes

Zoe M. Hale; Robert S. Marks; C. R. Lowe; Frank P. Payne

doi:10.1117/12.180745

28 July 1994 Single-mode tapered optical fiber immunosensor I: characterization with model analytes

Zoe M. Hale, Robert S. Marks, C. R. Lowe, Frank P. Payne

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180745
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A novel single mode tapered optical fiber loop biochemical sensor based on fluorescence spectroscopy has been developed. The fundamental fiber mode propagating through the tapered portion of the waveguide has evanescent fields which penetrate into the aqueous environment where the biochemical recognition event occurs. The model measurands were conjugated with a fluorescent dye, fluorescein isothiocyanate. When excited by the input laser light from the near end of the taper, generated fluorescence is coupled into the guided mode of the fiber and collected at the far end of the taper. Several radioactive and fluorescent quantification methods have been explored to determine the density of available binding sites immobilized on the fiber, and thus the ultimate sensitivity of the device. A generic avidin- biotin system has been tested as a model immunological diagnostic system. The high sensitivity of a single mode tapered loop device combined with a simple immobilization method provides a powerful tool for performing immunoassays.

Citation Download Citation

Zoe M. Hale, Robert S. Marks, C. R. Lowe, and Frank P. Payne "Single-mode tapered optical fiber immunosensor I: characterization with model analytes", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180745

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