Optimized streak-camera system: wide excitation range and extended time scale for fluorescence lifetime measurement

Urs Graf; Christof Buehler; Michael Betz; Herbert Zuber; M. Anliker

doi:10.1117/12.182729

17 August 1994 Optimized streak-camera system: wide excitation range and extended time scale for fluorescence lifetime measurement

Urs Graf, Christof Buehler, Michael Betz, Herbert Zuber, M. Anliker

Author Affiliations +

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182729
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A new versatile system for the measurement of time-resolved fluorescence emission spectra of biomolecules is presented. Frequency doubling and tripling of a Ti:Sapphire laser allows excitation over a wide wavelength range. The influence of increasing the spectral resolution on the time resolution has been investigated. System performance can be optimized for best resolution in the spectral or time domain, respectively. System performance can be optimized for best resolution in the spectral or time domain, respectively. The currently achieved temporal resolution is 6 psec, and the best spectral resolution is 3 nm. Long fluorescence decays can be resolved with optimal time resolution by way of taking into account the flyback of the streak camera. With the system described, the core complex ((alpha) (beta) )₃^APCL_C^8.9 of the phycobilisome from the photosynthetic cyanobacteria Mastigocladus laminosus has been analyzed. Lifetime analysis clearly demonstrated the influence of the linker polypeptide on the phycobiliprotein complex and the identity of native and reconstituted complex.

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Urs Graf, Christof Buehler, Michael Betz, Herbert Zuber, and M. Anliker "Optimized streak-camera system: wide excitation range and extended time scale for fluorescence lifetime measurement", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182729

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