Using 7-azaindole to probe condensed phase dynamics

R. L. Rich; F. Gai; Yeh Fong Chen; Jacob Petrich

doi:10.1117/12.182753

17 August 1994 Using 7-azaindole to probe condensed phase dynamics

R. L. Rich, F. Gai, Yeh Fong Chen, Jacob Petrich

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182753
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

In order to study experimentally the ultrafast (<1 picosecond to several hundreds of picoseconds) molecular dynamics of protein-protein interactions, an optical probe is required. Tryptophan has been the most widely used intrinsic optical probe of protein structure and dynamics. There are, however, two major problems attendant to the use of tryptophan, especially in fluorescence measurements. First, since tryptophan is a naturally-occurring amino acid there are often several tryptophans in a protein molecule whose emission must be distinguished. Second, the fluorescence decay of tryptophan itself in aqueous solution is nonexponential. We have consequently investigated alternatives to tryptophan. Our work has led us to the amino acid analog, 7-azatryptophan, and its chromophoric moiety, 7-azaindole.

Citation Download Citation

R. L. Rich, F. Gai, Yeh Fong Chen, and Jacob Petrich "Using 7-azaindole to probe condensed phase dynamics", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182753

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