Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques

Stefan Winter; Liviu M. Popa

doi:10.1117/12.198728

4 January 1995 Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques

Stefan Winter, Liviu M. Popa

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198728
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Dimers of the fluorescent dye Oxazole Yellow (YOYO and POPO) are used for high sensitive DNA detection in gel electrophoresis. Upon binding to DNA they show a 3000 to 5000 fold enhancement of fluorescence. The binding constant of those dimers to dsDNA is between 10⁸ M^-1 and 10⁹ M^-1. This is due to the dye's ability to bisintercalate between adjacent DNA basepairs. We investigated the occurring forms of intercalation of YOYO to dsDNA in solutions of different ionic strength by fluorescence lifetime methods.

Citation Download Citation

Stefan Winter and Liviu M. Popa "Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198728

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