Particle-enhanced total internal reflectance fluorescence

Victor P. Chu; Steven J. Zoha; Jim Davis; Suzette Chance

doi:10.1117/12.206011

3 April 1995 Particle-enhanced total internal reflectance fluorescence®

Victor P. Chu, Steven J. Zoha, Jim Davis, Suzette Chance

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206011
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

This paper presents work toward a retrofit of an aca^TMIV Discrete Clinical Analyzer (E. I. du Pont de Nemours and Company, Wilmington, DE) with a Particle Enhanced Total Internal Reflection Fluorescence technology. The DuPont aca^TM line of instruments was designed to perform highly accurate unit dose clinical chemistry assays. Introduction of a Total Reflectance Fluorescence optics into an aca^TMIV could provide capability to perform highly sensitive homogeneous immunoassays. Sensitivity and precision depended upon uniform immobilization of immunoglobulins or immunogens, representing examples of specific binding partners, onto an ionomeric material, acting as both solid support and wall material of the disposable reagent units called the `pack'. This study examines the uniformity of antibody coating onto various types of tough and flexible optical polymeric films including those made from the DuPont Surlyn^TM and Nucrel^TM polymers. A number of coating processes will also be described. Bioanalytical tools were developed to determine uniformity, quantity, and activity of antibody coated onto the `pack' film, thus allowing for optimization of polymer film type and process of antibody coating.

Citation Download Citation

Victor P. Chu, Steven J. Zoha, Jim Davis, and Suzette Chance "Particle-enhanced total internal reflectance fluorescence®", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206011

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KEYWORDS

Coating

Particles

Polymers

Adsorption

Luminescence

Statistical analysis

Proteins

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