31 January 1996Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs
Hugo J. van Staveren, Johan F. Beek, Cess W.J. Verlaan, Annie Edixhoven, Anne E. Saarnak, Dick Sterenborg, Theo M. de Reijke, Guy Brutel de la Riviere, Sharon L. Thomsen M.D., Martin J. C. van Gemert, Willem M. Star
Hugo J. van Staveren,1 Johan F. Beek,2 Cess W.J. Verlaan,2 Annie Edixhoven,3 Anne E. Saarnak,2 Dick Sterenborg,2 Theo M. de Reijke,2 Guy Brutel de la Riviere,1 Sharon L. Thomsen M.D.,4 Martin J. C. van Gemert,2 Willem M. Star1
1Daniel den Hoed Cancer Ctr. (Netherlands) 2Academic Medical Ctr. (Netherlands) 3Dijkzgt Hospital (Netherlands) 4Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The agent 5-aminolevulinic acid (5-ALA) can be an alternative drug in whole bladder wall (WBW) photodynamic therapy (PDT), as its good tumor selectivity and the short time skin photosensitivity after systemic administration are advantageous for clinical use. To determine the maximum drug and light doses for reversible normal tissue damage, a pre-clinical study was performed using an in vivo normal piglet bladder model. First, the kinetics of PpIX production in 2 pigs was determined in vitro after oral administration of 75 and 150 mg/kg ALA respectively. The concentration of PpIX in plasma, and erythrocytes was determined by reversed phase high-performance liquid chromatography (HPLC) and the maximum was reached at approximately equals 5 hours after the administration of ALA. This provided a guideline for the optimum interval between ALA administration and light application. Next, various ALA doses were either administered orally or instilled in the bladder and different light doses were applied. Bladder biopsies were taken at regular intervals and normal tissue damage was investigated histologically. Reversible tissue damage was obtained using 60 mg/kg of 5-ALA in combination with a light dose of 100 J cm-2 (non-scattered plus scattered 630 nm wavelength light) in the case of oral administration. In the case of intravesical instillation, a drug dose of 2.5 gram and a light dose of 100 J cm-2 are still too high to obtain reversible tissue damage.
Hugo J. van Staveren,Johan F. Beek,Cess W.J. Verlaan,Annie Edixhoven,Anne E. Saarnak,Dick Sterenborg,Theo M. de Reijke,Guy Brutel de la Riviere,Sharon L. Thomsen M.D.,Martin J. C. van Gemert, andWillem M. Star
"Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230951
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Hugo J. van Staveren, Johan F. Beek, Cess W.J. Verlaan, Annie Edixhoven, Anne E. Saarnak, Dick Sterenborg, Theo M. de Reijke, Guy Brutel de la Riviere, Sharon L. Thomsen M.D., Martin J. C. van Gemert, Willem M. Star, "Whole bladder wall photodynamic therapy using 5-ALA: an experimental study in pigs," Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.230951