Visualization and quantification of healthy and carious dentin structure using confocal laser scanning microscopy

Yuichi Kimura; Petra B. B. Wilder-Smith; Tatiana B. Krasieva; Anna-Marie A. Arrastia-Jitosho; Lih-Huei L. Liaw; Koukichi Matsumoto; Michael W. Berns

doi:10.1117/12.238754

23 April 1996 Visualization and quantification of healthy and carious dentin structure using confocal laser scanning microscopy

Yuichi Kimura, Petra B. B. Wilder-Smith, Tatiana B. Krasieva, Anna-Marie A. Arrastia-Jitosho, Lih-Huei L. Liaw, Koukichi Matsumoto, Michael W. Berns

Author Affiliations +

Proceedings Volume 2672, Lasers in Dentistry II; (1996) https://doi.org/10.1117/12.238754
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

In this study, a fluorescence technique was developed for visualization of dentin using confocal laser scanning microscopy (CLSM). Eighteen extracted human teeth were used: 13 showing no clinical signs of caries and 5 with visually apparent decay. Preliminary study: All teeth were horizontally sectioned to approx. 200 micrometers thickness and pre-treated as follows: no pretreatment; vacuum only; ultrasonication only; sodium hypochlorite (NaOCl) only; vacuum and NaOCl; ultrasonication and NaOCl; or vacuum, ultrasonication and NaOCl. Samples were stained with Rhodamine 123 fluorescent dye at a concentration of 10^-5 M in phosphate buffer saline for 1 to 24 hours. Caries study: Dentin surfaces, some with pre-existing caries, were visualized using CLSM. Most dentin tubules in sound dentin appeared open using CLSM, but most dentin tubules in carious dentin appeared closed or narrowed. Surface images obtained using CLSM were similar to those seen by SEM, but additional subsurface imaging was possible using CLSM at depth intervals of 1 micrometers to a depth of 30 - 50 micrometers . This technique shows good potential for non-invasive surface and subsurface imaging of dentin structures.

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Yuichi Kimura, Petra B. B. Wilder-Smith, Tatiana B. Krasieva, Anna-Marie A. Arrastia-Jitosho, Lih-Huei L. Liaw, Koukichi Matsumoto, and Michael W. Berns "Visualization and quantification of healthy and carious dentin structure using confocal laser scanning microscopy", Proc. SPIE 2672, Lasers in Dentistry II, (23 April 1996); https://doi.org/10.1117/12.238754

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