Neutron scattering in biology: from isotopic substitution to nuclear spin contrast variation

H. B. Stuhrmann

doi:10.1117/12.267880

27 February 1997 Neutron scattering in biology: from isotopic substitution to nuclear spin contrast variation

H. B. Stuhrmann

Proceedings Volume 2867, International Conference Neutrons in Research and Industry; (1997) https://doi.org/10.1117/12.267880
Event: Fifth International Conference on Applications of Nuclear Techniques: Neutrons in Research and Industry, 1996, Crete, Greece

Abstract

Biological applications of neutron scattering almost entirely rely on methods of isotropic substitution of hydrogen ¹H by its heavier isotope ²H. The exchange of water by heavy water D₂O has been most widely used for the characterization of the internal structure of complex particles, like viruses, mostly at low resolution. This method is known as contrast variation. A more detailed structural information is obtained when small components of a complex structure have a different isotopic composition. The structural studies on ribsomes are an example which will be discussed here. A further considerable enhancement of the contrast is observed when polarized neutrons are scattered by polarized proton spins.

Citation Download Citation

H. B. Stuhrmann "Neutron scattering in biology: from isotopic substitution to nuclear spin contrast variation", Proc. SPIE 2867, International Conference Neutrons in Research and Industry, (27 February 1997); https://doi.org/10.1117/12.267880

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