Breast cancer: in vitro measurements of native fluorescence

Wolfgang Lohmann; Rainer M. Bohle; Thomas Dreyer; Sabine Haas; Heike Wallenfels; Konrad Schwemmle; Wolf-Bernhard Schill

doi:10.1117/12.260813

6 December 1996 Breast cancer: in vitro measurements of native fluorescence

Wolfgang Lohmann, Rainer M. Bohle, Thomas Dreyer, Sabine Haas, Heike Wallenfels, Konrad Schwemmle, Wolf-Bernhard Schill

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260813
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Unfixed, HE stained cryosections of breast tissue obtained from 67 patients during surgery were illuminated with 395 - 440 nm and their fluorescence response as well as the 2- dimensional fluorophore distribution were measured. The histological evaluation of the same cryosection, illuminated as usual with a transmitted light obtained from a halogen lamp, revealed 9 patients with healthy tissue, 11 with benign epithelial hyperplasia, 4 with ductal carcinoma in situ, 35 with invasive ductal carcinoma, 7 with invasive lobular carcinoma, and one with invasive tubular carcinoma. A comparison between the fluorescence and the HE images shows that both match very nicely and that the fluorescence images are also characteristic for the different pathological condition of the biopsy sample. Moreover, benign tumors e.g. fibroadenomas, exhibit a fluorescence response different from cancer and healthy tissue.

Citation Download Citation

Wolfgang Lohmann, Rainer M. Bohle, Thomas Dreyer, Sabine Haas, Heike Wallenfels, Konrad Schwemmle, and Wolf-Bernhard Schill "Breast cancer: in vitro measurements of native fluorescence", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260813

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