Use of phosphorescent and fluorescent dyes for lifetime-based imaging within tissues

Alvin U. Chen; Eva Marie Sevick-Muraca

doi:10.1117/12.280239

18 August 1997 Use of phosphorescent and fluorescent dyes for lifetime-based imaging within tissues

Alvin U. Chen, Eva Marie Sevick-Muraca

Proceedings Volume 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II; (1997) https://doi.org/10.1117/12.280239
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The use of fluorescent and phosphorescent dyes has long been established in microscopy and bio-assays for rapid and accurate biochemical spectroscopy. In this work, we explore extension of these dyes for in vivo tissue spectroscopy. Using a theoretical model for the time-dependent light propagation through scattering media, we investigate the coupling of light propagation and re-emission kinetics for florescent and phosphorescent dyes delivered deep within tissues. Our results show that the use of phosphorescent dyes can provide spectroscopy or localization of specific tissue compartments, but may be information-poor for localized spectroscopy or imaging. While re-emitted fluorescence signals contain contributions owing to both light scattering and kinetics, they do contain the necessary information for localized spectroscopy.

Citation Download Citation

Alvin U. Chen and Eva Marie Sevick-Muraca "Use of phosphorescent and fluorescent dyes for lifetime-based imaging within tissues", Proc. SPIE 2979, Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model, and Human Studies II, (18 August 1997); https://doi.org/10.1117/12.280239

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