NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers

Frank N. Gellerich; Tobias Mueller; Shoko Nioka; Katrin Hertel; Wilhelm J. Schulte-Mattler; Stephan Zierz; Britton Chance

doi:10.1117/12.301043

1 January 1998 NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers

Frank N. Gellerich, Tobias Mueller, Shoko Nioka, Katrin Hertel, Wilhelm J. Schulte-Mattler, Stephan Zierz, Britton Chance

Author Affiliations +

Proceedings Volume 3194, Photon Propagation in Tissues III; (1998) https://doi.org/10.1117/12.301043
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Noninvasive measurement of changes in oxygenation of human skeletal muscle can be done with a dual-wavelength near infrared (NIR) spectrophotometer. This allows a noninvasive investigation of muscle mitochondria. An exercise protocol was developed to study the load dependent changes in oxygenation of m. vastus lateralis of myopathic patients. On a bicycle ergometer exercise was done periodically. One period consisted of 1.5 min exercise followed by 3 min rest. Work load in the first period was 20 W, and was increased by 10 W for each subsequent period until maximal work load was reached. In 12 healthy volunteers we observed oxygenation of muscle during periods of low work load (warm-up effect). During periods of high work load the muscle deoxygenated. The work load at transition from oxygenation to deoxygenation (deoxygenation threshold) in controls was 75 W. In 3 patients with myopathies, in addition to NIR- spectroscopy, function of mitochondria of specimen of m. vastus lateralis was investigated biochemically. Muscle fibers were skinned with saponin and investigated with high resolution respirometry and multiple substrate-inhibitor- titration. Mitochondrial function was impaired in patients who had abnormal findings in NIR spectroscopy.

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Frank N. Gellerich, Tobias Mueller, Shoko Nioka, Katrin Hertel, Wilhelm J. Schulte-Mattler, Stephan Zierz, and Britton Chance "NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers", Proc. SPIE 3194, Photon Propagation in Tissues III, (1 January 1998); https://doi.org/10.1117/12.301043

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