Optical coherence microscopy for the in-depth study of biological structures: system based on a parallel detection scheme

Emmanuel Beaurepaire; Philippe Gleyzes; Martial Lebec; Loic Blanchot; Herve Saint-Jalmes; Albert Claude Boccara

doi:10.1117/12.305380

16 April 1998 Optical coherence microscopy for the in-depth study of biological structures: system based on a parallel detection scheme

Emmanuel Beaurepaire, Philippe Gleyzes, Martial Lebec, Loic Blanchot, Herve Saint-Jalmes, Albert Claude Boccara

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Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305380
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

We present a new microscopy system based on the OCT principle, that uses a multiplexed lock-in detection scheme to generate a 2D head-on image in parallel without lateral scanning. Our 'full-field optical coherence microscopy' comprises a Michelson interferometer built with a polarizing beam splitter, and uses a photoelastic birefringence modulator to modulate the optical path difference between the two orthogonal polarizations. A novel signal processing method is used to achieve a demodulation in parallel on every pixel of a 256 X 256 CCD camera. A 840 nm electroluminescent diode with 20 micrometers coherence length is used to illuminate the field of view through the microscope objective lens. In-depth exploration of the sample is realized by changing the plane of focus. The lateral resolution of the images is limited by the camera pixel size and is 2 micrometers . The axial sectioning ability is approximately 8 micrometers . Having validated our setup on model samples, we now evaluate its performance on biological structures. As an example, images of onion cells from 50-400 micrometers below the surface are obtained in 1 s with 100 dB sensitivity.

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Emmanuel Beaurepaire, Philippe Gleyzes, Martial Lebec, Loic Blanchot, Herve Saint-Jalmes, and Albert Claude Boccara "Optical coherence microscopy for the in-depth study of biological structures: system based on a parallel detection scheme", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305380

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KEYWORDS

Modulation

Optical coherence tomography

Signal detection

Cameras

Objectives

Mirrors

Image resolution

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