Comparison of the effect of UV laser radiation and of a radiomimetic substance on chromatin

Irina Radulescu; Liliana Radu; Ruxandra Serbanescu; V. D. Nelea; C. Martin; Ion N. Mihailescu

doi:10.1117/12.312650

2 July 1998 Comparison of the effect of UV laser radiation and of a radiomimetic substance on chromatin

Irina Radulescu, Liliana Radu, Ruxandra Serbanescu, V. D. Nelea, C. Martin, Ion N. Mihailescu

Proceedings Volume 3405, ROMOPTO '97: Fifth Conference on Optics; (1998) https://doi.org/10.1117/12.312650
Event: ROMOPTO '97: Fifth Conference on Optics, 1997, Bucharest, Romania

Abstract

The damages of the complex of deoxyribonucleic acid (DNA) and proteins from chromatin, produced by the UV laser radiation and/or by treatment with a radiomimetic substance, bleomycin, were compared. The laser radiation and bleomycin effects on chromatin structure were determined by the static and dynamic fluorimetry of chromatin complexes with the DNA specific ligand-- proflavine and by the analysis of tryptophan chromatin intrinsic fluorescence. Time resolved spectroscopy is a sensitive technique which allows to determine the excited state lifetimes of chromatin--proflavine complexes. Also, the percentage contributions to the fluorescence of proflavine, bound and unbound to chromatin DNA, were evaluated. The damages produced by the UV laser radiation on chromatin are similar with those of radiomimetic substance action and consists in DNA and proteins destruction. The DNA damage degree has been determined. The obtained results may constitute some indications in the laser utilization in radiochimiotherapy.

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Irina Radulescu, Liliana Radu, Ruxandra Serbanescu, V. D. Nelea, C. Martin, and Ion N. Mihailescu "Comparison of the effect of UV laser radiation and of a radiomimetic substance on chromatin", Proc. SPIE 3405, ROMOPTO '97: Fifth Conference on Optics, (2 July 1998); https://doi.org/10.1117/12.312650

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KEYWORDS

Luminescence

Ultraviolet radiation

Proteins

Radiation effects

Laser irradiation

Time resolved spectroscopy

Deconvolution

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