Detection of bacteria from a cecal anaerobic competitive exclusion culture with an immunoassay electrochemiluminescence sensor

Ross C. Beier; Colin R. Young; Larry H. Stanker

doi:10.1117/12.335773

12 January 1999 Detection of bacteria from a cecal anaerobic competitive exclusion culture with an immunoassay electrochemiluminescence sensor

Ross C. Beier, Colin R. Young, Larry H. Stanker

Author Affiliations +

Proceedings Volume 3544, Pathogen Detection and Remediation for Safe Eating; (1999) https://doi.org/10.1117/12.335773
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

A competitive exclusion (CE) culture of chicken cecal anaerobes has been developed and used in this laboratory for control of Salmonella typhimurium in chickens. The CE culture consists of 29 different species of micro-organisms, and is known as CF3. Detection of one of the CF3 bacteria, Eubacteria, and S. typhimurium were demonstrated using a commercial immunomagnetic (IM) electrochemiluminescence (ECL) sensor, the ORIGEN^R Analyzer. Analysis was achieved using a sandwich immunoassay. Bacteria were captured on antibody- conjugated 280 micron sized magnetic beads followed by binding of reporter antibodies labelled with ruthenium (II) tris(dipyridyl) chelate [Ru(bpy)₃²⁺]. The magnetic beads were then trapped on an electrode in the reaction cell of the ORIGEN^R Analyzer by a magnet, and the ECL was evoked from Ru(bpy)₃²⁺ on the tagged reporter antibodies by an electrical potential at the electrode. Preliminary IM-ECL assays with Eubacteria yielded a detection limit of 10⁵ cfu/mL. Preliminary IM-ECL assays with S. typhimurium yielded a similar detection limit of 10⁵ cfu/mL.

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Ross C. Beier, Colin R. Young, and Larry H. Stanker "Detection of bacteria from a cecal anaerobic competitive exclusion culture with an immunoassay electrochemiluminescence sensor", Proc. SPIE 3544, Pathogen Detection and Remediation for Safe Eating, (12 January 1999); https://doi.org/10.1117/12.335773

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