Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm

Jan Slavik; Edvard Lanz

doi:10.1117/12.336822

19 January 1999 Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm

Jan Slavik, Edvard Lanz

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336822
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

The reliable and widely used pH-sensitive indicator BCECF (2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) can also be employed in a classical confocal laser scanning microscopes using 488 nm argon laser excitation line. The intracellular pH maps based on the fluorescence emission ratio tally with those obtained by other techniques.

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Jan Slavik and Edvard Lanz "Estimation of intracellular pH in argon laser scanning confocal microscopes with a laser line of 488 nm", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336822

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KEYWORDS

Microscopes

Argon ion lasers

Luminescence

Confocal microscopy

Laser scanners

Yeast

Proteins

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