Improved fluorophores for zinc biosensing using carbonic anhydrase

Richard B. Thompson; Badri P. Maliwal; Hui-Hui Zeng

doi:10.1117/12.346738

21 April 1999 Improved fluorophores for zinc biosensing using carbonic anhydrase

Richard B. Thompson, Badri P. Maliwal, Hui-Hui Zeng

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346738
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Previously, we had shown that the zinc-dependent binding of certain fluorescent aryl sulfonamide inhibitors could be used with apo-carbonic anhydrase II to transduce the level of free zinc as a change in the fluorescence of the inhibitor. While inhibitors such as dansylamide, ABD-M, and ABD-N made possible quantitation of free zinc in the picomolar range with high selectivity, they have only modest absorbance which limits their utility. We describe here the synthesis and properties of two new probes, Dapoxyl sulfonamide and BTCS, and their use in zinc biosensing. Dapoxyl sulfonamide exhibits a dramatic increase and blue shift in its emission upon binding to holo-carbonic anhydrase II, as well as a twenty-fold increase in lifetime: it is thus well suited for quantitating free Zn(II) down to picomolar ranges. The anisotropy of BTCS increases five-fold binding to the holoprotein making this probe well suited for anisotropy-based determination of zinc.

Citation Download Citation

Richard B. Thompson, Badri P. Maliwal, and Hui-Hui Zeng "Improved fluorophores for zinc biosensing using carbonic anhydrase", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346738

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