Laser-scan cytometry: a new tool for clinical diagnostics

Holger K. Maerz; Adolf Baumgartner; Joerg Hambsch; Bert Hennig; Michael Nuesse; Thomas Schmid; Peter Schneider; Rainer Zotz; Attila Tarnok

doi:10.1117/12.346761

21 April 1999 Laser-scan cytometry: a new tool for clinical diagnostics

Holger K. Maerz, Adolf Baumgartner, Joerg Hambsch, Bert Hennig, Michael Nuesse, Thomas Schmid, Peter Schneider, Rainer Zotz, Attila Tarnok

Author Affiliations +

Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346761
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The common usage of flow cytometry (FCM) in research and clinical diagnostic is limited by the lack visualizing the fluorescence labelled cells. The Laser Scanning Cytometer (LSC) enables multicolor cytometric measurements on a slide featuring relocation of single cells for further investigation via brightfield and fluorescence microscopy. Additionally, it is possible to capture these images for documentation. In a FISH application, the LSC was successfully used for automated scoring techniqeus for evaluating the frequency of aneuploid sperm in humans and mice. In just 30 minutes, we were able to acquire more than 15,000 sperms, a task which normally takes more than a day. After relocation, genetic defects were identified and confirmed via fluorescence microscopy. In an on going study, we investigate via the LSC the remain of a new radiopaque material for high resolution echocardiography in the blood circulation. At first the result exhibited that the radiopaque material is endocysed by leukocytes just after application but is still detectable via echocardiography for up to 40 minutes. In conclusion, with the additional data acquisition by the LSC, it is possible to perform further detailed information from very small samples. Therefore, we are working up to now on developing new methods to introduce the LSC in our clinical diagnostic of neonates undergoing cardiac surgery.

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Holger K. Maerz, Adolf Baumgartner, Joerg Hambsch, Bert Hennig, Michael Nuesse, Thomas Schmid, Peter Schneider, Rainer Zotz, and Attila Tarnok "Laser-scan cytometry: a new tool for clinical diagnostics", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346761

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