Two-photon excited imaging of photosensitizers in tissues

Mariah L. Coleno; Vincent P. Wallace; Chung-Ho Sun; Andrew K. Dunn; Michael W. Berns; Bruce J. Tromberg

doi:10.1117/12.349217

1 June 1999 Two-photon excited imaging of photosensitizers in tissues

Mariah L. Coleno, Vincent P. Wallace, Chung-Ho Sun, Andrew K. Dunn, Michael W. Berns, Bruce J. Tromberg

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Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349217
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Two-photon microscopy (TPM) is a non-invasive biological imaging technique that can be used to selectively image cellular activity and photosensitizer (PS) localization within highly scattering epithelial tissues at depths of approximately 200 micrometer with submicron resolution. The principal objective of this study was to develop a model system for understanding the impact of photodynamic therapy on cellular and extracellular matrix remodeling in biological tissues. An artificial tissue model (RAFT) composed of collagen, embedded fibroblasts, and macrophage cells has been developed for this purpose. TPM is utilized to monitor extracellular matrix remodeling following PDT by imaging collagen/elastin autofluorescence. Selective uptake of photosensitizers by specific cellular components in the matrix can also be visualized by TPM.

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Mariah L. Coleno, Vincent P. Wallace, Chung-Ho Sun, Andrew K. Dunn, Michael W. Berns, and Bruce J. Tromberg "Two-photon excited imaging of photosensitizers in tissues", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349217

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KEYWORDS

Tissues

Collagen

Luminescence

Photodynamic therapy

Wound healing

Two photon imaging

Microscopes

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