Detection of prostaglandin H synthase (cyclooxygenase) -1 and -2 expression in single cancer cells by confocal Raman microspectroscopy

Kasem Nithipatikom; Michael J. McCoy; Andre Kajdacsy-Balla; Sushma Kaul; Paul F. Lindholm; Sharon R. Hawi; Sunant Rochanakij; Fran Adar; William B. Campbell

doi:10.1117/12.345410

9 April 1999 Detection of prostaglandin H synthase (cyclooxygenase) -1 and -2 expression in single cancer cells by confocal Raman microspectroscopy

Kasem Nithipatikom, Michael J. McCoy, Andre Kajdacsy-Balla, Sushma Kaul, Paul F. Lindholm, Sharon R. Hawi, Sunant Rochanakij, Fran Adar, William B. Campbell

Author Affiliations +

Proceedings Volume 3608, Biomedical Applications of Raman Spectroscopy; (1999) https://doi.org/10.1117/12.345410
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Prostaglandin H synthase (PGHS), commonly known as cyclooxygenase (COX), is the enzyme involved in the synthesis of biologically active prostanoids from arachidonic acid. There are two PGHS isozymes with identical functions, similar sizes and similar structures. PGHS-1 is constitutively expressed in most mammalian cells where as PGHS-2 is induced by various agents. PGHS-2 expression was increased with inflammation, mitogenesis, and some types of cancer. We have developed a method to simultaneously detect PGHS-1 and PGHS-2 in single cancer cells by using specific antibodies, surface-enhanced Raman scattering, and confocal Raman microspectroscopy. Cells were plated, cultured, incubated for immunocomplexation, and detected directly in the wells of a multiwell plate. The expression and localization of PGHS-1 and -2 in single malignant human hepatocytes was detected and compared to normal cells. Furthermore, the correlation between the expression of PGHS and the invasiveness of cancer cells was investigated in PC- 3 cell sublines.

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Kasem Nithipatikom, Michael J. McCoy, Andre Kajdacsy-Balla, Sushma Kaul, Paul F. Lindholm, Sharon R. Hawi, Sunant Rochanakij, Fran Adar, and William B. Campbell "Detection of prostaglandin H synthase (cyclooxygenase) -1 and -2 expression in single cancer cells by confocal Raman microspectroscopy", Proc. SPIE 3608, Biomedical Applications of Raman Spectroscopy, (9 April 1999); https://doi.org/10.1117/12.345410

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KEYWORDS

Cancer

Raman spectroscopy

Confocal microscopy

Imaging spectroscopy

Prostate cancer

Silver

Colorectal cancer

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