Next fundamental steps for optical DNA biosensors

Lisa Henke; James H. Watterson; Ulrich J. Krull

doi:10.1117/12.382028

11 April 2000 Next fundamental steps for optical DNA biosensors

Lisa Henke, James H. Watterson, Ulrich J. Krull

Proceedings Volume 3913, In-Vitro Diagnostic Instrumentation; (2000) https://doi.org/10.1117/12.382028
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Methods for the detection of pathogenic organisms and genetic mutations have evolved to use the selective recognition chemistry offered by nucleic acid hybridization. The immobilization of single-stranded DNA as a 'receptor' onto various solid surfaces that are suitable for optical sensor development is a fundamental issue that determines aspects of selectivity, sensitivity and speed. This manuscript presents an evaluation of various immobilization strategies to attach ssDNA to surfaces, the control of such immobilization to design optical and thermodynamic properties of monolayer films, and suggests approaches for optimization of analytical figures of merit and the potential for quantitative analysis.

Citation Download Citation

Lisa Henke, James H. Watterson, and Ulrich J. Krull "Next fundamental steps for optical DNA biosensors", Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); https://doi.org/10.1117/12.382028

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