Probing single-molecule orientations in confined sample regions with near-field scanning optical microscopy

Christopher W. Hollars; Robert C. Dunn

doi:10.1117/12.383350

21 April 2000 Probing single-molecule orientations in confined sample regions with near-field scanning optical microscopy

Christopher W. Hollars, Robert C. Dunn

Author Affiliations +

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383350
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Near-field scanning optical microscopy (NSOM) is utilized to study the 3D orientation of fluorescent probe molecules doped into thin polymer films and lipid monolayers. A simplified model of the fields near the NSOM tip aperture is used to simulate the single molecule near-field fluorescence features and extract the orientation of the probe molecules in the films. These measurements are particular useful for semi-ordered systems such as lipid monolayers that are often utilized as models of biological membranes. These single molecule measurements can reveal new features and provide details previously hidden in bulk methods that average over large ensembles of molecules.

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Christopher W. Hollars and Robert C. Dunn "Probing single-molecule orientations in confined sample regions with near-field scanning optical microscopy", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383350

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KEYWORDS

Molecules

Near field scanning optical microscopy

Luminescence

Near field

Polymethylmethacrylate

Mica

Polarization

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