PDT-induced apoptosis: what are the critical molecular targets

Nancy L. Oleinick; Irina Belichenko; Song-mao Chiu; Minh C. Lam; Rachel L. Morris; Liang-yan Xue

doi:10.1117/12.424444

9 April 2001 PDT-induced apoptosis: what are the critical molecular targets

Nancy L. Oleinick, Irina Belichenko, Song-mao Chiu, Minh C. Lam, Rachel L. Morris, Liang-yan Xue

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424444
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Early molecular damages have been studied in a series ofhuman tumor and rodent cell lines following photodynamic therapy (PDT) sensitized by the silicon phthalocyanine Pc 4. Pc 4 preferentially localizes in mitochondria, and upon photoirradiation, immediate photodamage to the anti-apoptotic oncoprotein Bcl-2 is observed. The loss ofthe native 26-kDa protein, as evidenced by western blot analysis, is accompanied by the generation of a 23 -kDa fragment from a small portion of the molecules as well as a variety ofhigher molecular weight protein bands indicative ofphotochemical crosslinking ofBcl-2 to itself, to (pro-apoptotic) homologs, or to other nearby proteins. The changes in Bcl-2 are apparent immediately upon exposure ofPc 4-loaded cells to activating red light, occur in the cold, and are not dependent upon caspase-3 or other proteases. Crosslinking is also observed for the intermediate filament protein vimentin. The results implicate Bcl-2 (and perhaps vimentin) as important molecular targets that lead to apoptosis in Pc 4-PDT-treated cells.

Citation Download Citation

Nancy L. Oleinick, Irina Belichenko, Song-mao Chiu, Minh C. Lam, Rachel L. Morris, and Liang-yan Xue "PDT-induced apoptosis: what are the critical molecular targets", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424444

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