Heat properties of an integrated micro PCR vessel

Zhan Zhao; Dafu Cui; Zhongyao Yu; Li Wang; Shanhong Xia; Zheng Cui

doi:10.1117/12.440202

14 September 2001 Heat properties of an integrated micro PCR vessel

Zhan Zhao, Dafu Cui, Zhongyao Yu, Li Wang, Shanhong Xia, Zheng Cui

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440202
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

The PCR amplification is based on multiple temperature cycles of DNA synthesis; each includes denaturation of the template, annealing of the primers to complementary sites in the template and primer extension. The key technique of PCR amplification is the heating control in design and fabrication of its chip form. The specifications of the chip are heat properties. In this paper the heat properties of a micro PCR vessel integration heater and temperature sensor was introduced. The temperature distribution of the vessel was simulated with software tool IntelliSuite. The temperatures cycles were measured and the time response of the chip was discussed. It is found that the integrate micro vessel is a very useful tool not only for DNA synthesis but also as a biochemical reactor for many other biological and chemical analyses.

Citation Download Citation

Zhan Zhao, Dafu Cui, Zhongyao Yu, Li Wang, Shanhong Xia, and Zheng Cui "Heat properties of an integrated micro PCR vessel", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440202

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