Optical coherence imaging of rat tumor spheroids

Ping Yu; M. Mustata; William Robert Headley; David D. Nolte; J. J. Turek; Paul M. W. French

doi:10.1117/12.470485

14 June 2002 Optical coherence imaging of rat tumor spheroids

Ping Yu, M. Mustata, William Robert Headley, David D. Nolte, J. J. Turek, Paul M. W. French

Proceedings Volume 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI; (2002) https://doi.org/10.1117/12.470485
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We report the first results of optical coherence imaging (OCI) of rat tumor spheroids. OCI is a full-frame variant of optical coherence tomography (OCT). The coherent image spatially modulates a high-sensitivity dynamic holographic film composed of a photorefractive quantum well (PRQW). Full-frame readout out of the hologram is observed in real time on a video camera. This system may be considered generally as a video camera with a coherence filter on the lens. Tumor spheroids are small (100-1000 m) balls of tumor cells that are cultured in vitro. Larger spheroids have increasingly complex inner structure. Necrosis and calcification form and expand, reminiscent of structure in malignant cysts in human tumors. In addition, rafts of tumor cells become separated by fluid-filled voids. These features are within the resolution limit of the OCI system, and produce highly structured coherent images.

Citation Download Citation

Ping Yu, M. Mustata, William Robert Headley, David D. Nolte, J. J. Turek, and Paul M. W. French "Optical coherence imaging of rat tumor spheroids", Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); https://doi.org/10.1117/12.470485

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