Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination

Michael I. Shribak; Rudolf Oldenbourg

doi:10.1117/12.467840

15 May 2002 Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination

Michael I. Shribak, Rudolf Oldenbourg

Author Affiliations +

Proceedings Volume 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX; (2002) https://doi.org/10.1117/12.467840
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The article presents a new design of a polarization microscope with a scanning liquid crystal aperture. The scanning device is based on the earlier reported Pol-Scope technique and includes a liquid crystal universal compensator. It is mounted in the front focal plane of the high numerical aperture condenser lens on the microsccope. By occluding different portions of the aperture, an oblique beam of variable tilt angle and azimuth is created for illuminating the specimen. Birefringence measurements are recorded for different mask configurations and results are evaluated to determine the retardance magnitude, azimuth and direction of optic axis of the specimen. We report measurements using small calcite crystals that confirm our theoretical predictions.

Citation Download Citation

Michael I. Shribak and Rudolf Oldenbourg "Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination", Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); https://doi.org/10.1117/12.467840

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available