Characterization of biological structures with nonlinear chemical imaging nanomicroscopy

Richard D. Schaller; Justin Johnson; Kevin R. Wilson; Lynn Lee; Louie Haber; Richard J. Saykally

doi:10.1117/12.461388

1 April 2002 Characterization of biological structures with nonlinear chemical imaging nanomicroscopy

Richard D. Schaller, Justin Johnson, Kevin R. Wilson, Lynn Lee, Louie Haber, Richard J. Saykally

Proceedings Volume 4633, Commercial and Biomedical Applications of Ultrafast and Free-Electron Lasers; (2002) https://doi.org/10.1117/12.461388
Event: High-Power Lasers and Applications, 2002, San Jose, California, United States

Abstract

Techniques of coherent nonlinear spectroscopy (second harmonic generation and third harmonic generation) are combined with near-field scanning optical microscopy for imaging selected chemical and physical environments in biological matter on a nanoscopic scale. Resonant enhancement of nonlinear signals is utilized as a method of producing chemically selective contrast while the order of the process provides environmental selectivity. Systems studied include natural killer cells and erythrocytes.

Citation Download Citation

Richard D. Schaller, Justin Johnson, Kevin R. Wilson, Lynn Lee, Louie Haber, and Richard J. Saykally "Characterization of biological structures with nonlinear chemical imaging nanomicroscopy", Proc. SPIE 4633, Commercial and Biomedical Applications of Ultrafast and Free-Electron Lasers, (1 April 2002); https://doi.org/10.1117/12.461388

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