Continuous bioaerosol monitoring using UV excitation fluorescence

Jay D. Eversole; C. S. Scotto; Mel Spence; Anthony J. Campillo

doi:10.1117/12.525515

19 November 2003 Continuous bioaerosol monitoring using UV excitation fluorescence

Jay D. Eversole, C. S. Scotto, Mel Spence, Anthony J. Campillo

Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.525515
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

We describe an instrument developed to monitor the biological fraction of ambient aerosol. This device simultaneously sizes individual particles in an air stream, and measures their total fluorescence following excitation at 266 nm. Recent results of single blind outdoor tests carried out in Alberta, Canada are described. In these, aerosols were generated containing four different types of biological material: ova albumin, MS-2 phage, Erwinia herbicola vegetative cells and Bacillus subtilis spores. Results indicate a probability of detection of 87% was achieved for target aerosol concentrations as low as a few (1-5) P particles/liter. Absolute quantitative detection efficiencies for individual bioaerosols were at least 70%. During the tests, nonbiological aerosols were also released and found not to generate any significant fluorescent signals.

Citation Download Citation

Jay D. Eversole, C. S. Scotto, Mel Spence, and Anthony J. Campillo "Continuous bioaerosol monitoring using UV excitation fluorescence", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.525515

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