Study of the intensity ratio from the characteristics of autofluorescence spectra of the human colorectal tissue

Tianyun Zhao; Teck-Chee Chia; Cheong Hoong Diong

doi:10.1117/12.474747

17 February 2003 Study of the Intensity Ratio from the Characteristics of Autofluorescence Spectra of the Human Colorectal Tissue

Tianyun Zhao, Teck-Chee Chia, Cheong Hoong Diong

Author Affiliations +

Proceedings Volume 4833, Applications of Photonic Technology 5; (2003) https://doi.org/10.1117/12.474747
Event: Applications of Photonic Technology 5, 2002, Quebec City, Canada

Abstract

Autofluorescence is widely investigated as a sensitive method in early diagnosis of diseased tissue, but most measurements of tissue autofluorescence were performed with only a single excitation wavelength. This work is aim to optimize the excitation wavelength or emission wavelength bands for fluorescence spectroscopy for better clinical diagnostic accuracy. The autofluorescence spectra of colorectal tissue were studied over a wide excitation range (350-600 nm). The excitation 350 nm and 470 nm were identified as effective excitation wavelength for inducing tissue autofluorescence. The ratio of emission peak intensity at 470 nm versus 610 nm excited by 350 nm and the ratio of emission peak intensity at 510 nm versus 610 nm excited by 470 nm were found as good indications to distinguish normal and diseased tissue. The observed peaks of the fluorescence spectra were also compared with some well known tissue fluorophores.

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Tianyun Zhao, Teck-Chee Chia, and Cheong Hoong Diong "Study of the Intensity Ratio from the Characteristics of Autofluorescence Spectra of the Human Colorectal Tissue", Proc. SPIE 4833, Applications of Photonic Technology 5, (17 February 2003); https://doi.org/10.1117/12.474747

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KEYWORDS

Tissues

Luminescence

Tissue optics

Collagen

Cancer

Colorectal cancer

Diagnostics

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