Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe

Da Xing; Juan Wang; Yonghong He

doi:10.1117/12.482963

12 September 2002 Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe

Da Xing, Juan Wang, Yonghong He

Proceedings Volume 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment; (2002) https://doi.org/10.1117/12.482963
Event: Photonics Asia, 2002, Shanghai, China

Abstract

In this paper, we report a rapid imaging method for oxygen free radicals produced in photodynamic therapy (PDT) in vivo using chemiluminescence probe. The mechanism for PDT involves singlet oxygen (102)generated by energy transfer from photosensitizers. '02 can react with FCLA, which is a specific chemiluminescence probe for detecting 102 and superoxide (02). The reaction of FCLA (Fluoresceinyl Cypridina Luminescent Analog) and 102can give emission with peak wavelength at about 532 nm. In the present study, FCLA was chosen as an optical reporter of 102produced from photosensitization reaction of Hematoporphyrin Derivative (HpD) in model solution and in nude mice with transplanted mammary cancer. Photosensitized chemiluminescence from the reaction of FCLA with 102was detected by a highly sensitive Intensified Charge-Coupled Device (ICCD) detector. The chemiluminescence was markedly inhibited by the addition of 10 mmol/L sodium azide (NaN3) to the model solution and minor effects were observed at the addition of 1 0 imol/L superoxide dismutase (SOD), 20 mmol/L mannitol and I 00 tg/mL catalase, respectively, thus indicating that '02 generation from photosensitization reaction mainly results in the light emission. Experiments in vivo with tumor-bearing mouse showed a clear chemiluminescence image of tumor.

Citation Download Citation

Da Xing, Juan Wang, and Yonghong He "Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); https://doi.org/10.1117/12.482963

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KEYWORDS

Chemiluminescence

Tumors

Oxygen

Photodynamic therapy

In vivo imaging

Sensors

Visible radiation

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