CARS microscopy with dark field excitation

Christoph Heinrich; Stefan Bernet; Monika Ritsch-Marte

doi:10.1117/12.499634

9 October 2003 CARS microscopy with dark field excitation

Christoph Heinrich, Stefan Bernet, Monika Ritsch-Marte

Author Affiliations +

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.499634
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We use coherent anti-Stokes Raman scattering (CARS) for functional imaging in microscopy. In contrast to other methods, excitation and detection are performed in a wide-field (non-confocal) setup, similar to a combination of dark field and epi-fluorescence microscopy. Thus, imaging of the whole sample is performed at once, i.e. without scanning, which promises the possibility of fast microscopy with vibrational contrast. The use of a nanosecond laser system rather than typically used pico- or femtosecond systems facilitates a high spectroscopic resolution for various organic substances.

Citation Download Citation

Christoph Heinrich, Stefan Bernet, and Monika Ritsch-Marte "CARS microscopy with dark field excitation", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.499634

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