Fluorescence measurements of activity associated with a molecularly imprinted polymer imprinted to dipicolinic acid

John Anderson; Dmitry Pestov; Robert L. Fischer; Stanley Webb; Gary C. Tepper

doi:10.1117/12.519480

8 March 2004 Fluorescence measurements of activity associated with a molecularly imprinted polymer imprinted to dipicolinic acid

John Anderson, Dmitry Pestov, Robert L. Fischer, Stanley Webb, Gary C. Tepper

Author Affiliations +

Proceedings Volume 5269, Chemical and Biological Point Sensors for Homeland Defense; (2004) https://doi.org/10.1117/12.519480
Event: Optical Technologies for Industrial, Environmental, and Biological Sensing, 2003, Providence, RI, United States

Abstract

Steady state and lifetime fluorescence measurements were acquired to measure the binding activity associated with molecularly imprinted polymer (MIP) microparticles imprinted to dipicolinic acid. Dipicolinic acid is a unique compound associated with the sporulation phase of spore-forming bacteria (e.g., genus Bacillus and Clostridium). Vinylic monomers were polymerized in a dimethylformamide solution containing the dipicolinic acid as a template. The resulting MIP was then pulverized and size selected into small microscale particles. Samplers were adapted incorporating the MIP particles within a dialyzer (500 MW). Tests were run on replicate samples of biologically active cultures representing both stationary phase and sporulation post fermentation products in standard media. The permeability of the membrane permitted diffusion of lighter molecular weight constituents from media effluents to enter the dialyzer chamber and contact the MIP. Extractions of the media were measured using steady state and lifetime fluorescence. Results showed dramatic steady state fluorescence changes as a function of excitation, emission and intensity and an estimated lifetime of 5.8 ns.

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John Anderson, Dmitry Pestov, Robert L. Fischer, Stanley Webb, and Gary C. Tepper "Fluorescence measurements of activity associated with a molecularly imprinted polymer imprinted to dipicolinic acid", Proc. SPIE 5269, Chemical and Biological Point Sensors for Homeland Defense, (8 March 2004); https://doi.org/10.1117/12.519480

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