Photodynamic therapy for the treatment of metastatic lesions in bone: studies in rat and porcine models

S. Burch; Stuart K. Bisland; Jeff Siewerdsen; Arjen Bogaards; Douglas Moseley; A. Yee; J. Finkelstein; Brian Wilson

doi:10.1117/12.548174

14 June 2004 Photodynamic therapy for the treatment of metastatic lesions in bone: studies in rat and porcine models

S. Burch, Stuart K. Bisland, Jeff Siewerdsen, Arjen Bogaards, Douglas Moseley, A. Yee, J. Finkelstein, Brian Wilson

Proceedings Volume 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII; (2004) https://doi.org/10.1117/12.548174
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

This study represents the first reported use of photodynamic therapy in bone and specifically, as a treatment for spinal metastases. A metastatic model in rat confirmed the efficacy of benzoporphyrin derivative-monoacid-mediated PDT for treating lesions within the spine and appendicular bone. Fluorimetry confirmed the selective accumulation of drug into the tumor(s) at 3 hours post-injection. 48 hrs post light delivery into the vertebral body of the rat spine loss of bioluminescent signal and histological analyses of sectioned spine confirmed MT-1 tumor cell kill in vivo as previously confirmed in vitro using an established cell viability assay. Porcine vertebrae provided a model comparable to that of human for light propagation and PDT response. Light measurements were recorded at 2.5 mm increments as the detector probe was retracted out of the vertebral body away from a diffusing fiber at 70-90° planar angle to it. At 30 minutes or 1hr post BPD-MA administration (6 mg/m²), light (648 J, 150 mW/cm, 690 nm) was delivered to vertebrae L1 and/or L2. Vertebrae were harvested and sectioned for histology 48 hrs following PDT. Light propagation was plotted as distance (μm) from the emitting source. Results support the application of PDT to the treatment of primary or metastatic lesions within bone.

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S. Burch, Stuart K. Bisland, Jeff Siewerdsen, Arjen Bogaards, Douglas Moseley, A. Yee, J. Finkelstein, and Brian Wilson "Photodynamic therapy for the treatment of metastatic lesions in bone: studies in rat and porcine models", Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); https://doi.org/10.1117/12.548174

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