Modulation of quantum dot photoemission based on fluorescence resonance energy transfer to a photochromic dye acceptor

Igor L. Medintz; Aaron R. Clapp; Scott A. Trammel; Hedi M. Mattoussi

doi:10.1117/12.570681

29 December 2004 Modulation of quantum dot photoemission based on fluorescence resonance energy transfer to a photochromic dye acceptor

Igor L. Medintz, Aaron R. Clapp, Scott A. Trammel, Hedi M. Mattoussi

Author Affiliations +

Proceedings Volume 5593, Nanosensing: Materials and Devices; (2004) https://doi.org/10.1117/12.570681
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

We demonstrate the use of a photochromic dye to achieve fluorescence resonance energy transfer (FRET) modulation between a QD donor and the dye acceptor brought in close proximity in a selfassembled QD-protein-dye conjugate. The E. coli maltose binding protein (MBP) appended on its C-terminal with an oligohistidine attachment domain, immobilized onto CdSe-ZnS core-shell QDs was labeled with a sulfo-N-hydroxysuccinimide activated photochromic BIPS molecule (1',3-dihydro-1'-(2-carboxyethyl)-3,3-dimethyl-6-nitrospiro[2H-1-benzopyran-2,2'-(2H)-indoline]). Two different dye-to-MBP-protein ratios of 1:1 and 5:1 were used. The ability of MBP-BIPS to modulate QD photoluminescence was tested by switching BIPS from the colorless spiropyran (SP) to the colored merocyanine (MC) using irradiation with white light (>500 nm) or with UV light (~365 nm), respectively. QDs surrounded by ~20 MBP-BIPS with a dye to protein ratio of 1 showed ~25% loss in their photoemission with consecutive repeated switches, while QDs surrounded by ~20 MBP-BIPS with BIPS to MBP ratio of 5 produced a substantially more pronounced rate of FRET where the QD emission was quenched by ~60%. This result suggests the possibility of using QD-protein conjugates to assemble reversible FRET nanoassemblies where the QD emission can be controlled by changing the properties of the acceptors dyes bound to the protein.

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Igor L. Medintz, Aaron R. Clapp, Scott A. Trammel, and Hedi M. Mattoussi "Modulation of quantum dot photoemission based on fluorescence resonance energy transfer to a photochromic dye acceptor", Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); https://doi.org/10.1117/12.570681

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