Cytological effect of laser-irradiated peanut seeds and induction of mutation

Lin Ou; Rong Chen; Tong Lin; Buhong Li; Weijian Zhuang

doi:10.1117/12.572813

18 January 2005 Cytological effect of laser-irradiated peanut seeds and induction of mutation

Lin Ou, Rong Chen, Tong Lin, Buhong Li, Weijian Zhuang

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.572813
Event: Photonics Asia, 2004, Beijing, China

Abstract

In this present paper, dry seeds of peanut of cytological effects and agro-character in F₁ generation have been studied for YAG laser-irradiated at 1060 nm. With transmission electron microscope, the effects of ultrastructure of plumule cell were observed. There were difference in the cell on membrane system, fatbody and nucleus, especially in proteinbody, and there was chromosomal aberration of peanut root tip cell induced with laser 35.84w/cm² power density. With irradiating time added from3s to 8s the rate of aberration increased from 0.16% up to 0.53%, and the types of chromosomal aberration also increased. Many aberration types, such as earlier separated mitosis metaphase, variability of number, sticky, non-equal division, polypolarity division, nucleus protrudes to cytoplasm and so on were found in 8s treatment but not in 3s. However, the dose of 8s treatment leads to lethal dose according to the seeds sprouting and growing. The seeds with 3s treatment, mine stem height, total pods per plant, mature pods per plant and weight per plant were more than the seeds unirradiated.

Citation Download Citation

Lin Ou, Rong Chen, Tong Lin, Buhong Li, and Weijian Zhuang "Cytological effect of laser-irradiated peanut seeds and induction of mutation", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.572813

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available