Enzyme immunoassay by dynamic enhanced vibrational spectroscopy of the enzyme reaction product

Haiying Zhao; Xiaoming Dou

doi:10.1117/12.575874

18 January 2005 Enzyme immunoassay by dynamic enhanced vibrational spectroscopy of the enzyme reaction product

Haiying Zhao, Xiaoming Dou

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.575874
Event: Photonics Asia, 2004, Beijing, China

Abstract

This paper reports a kind of application of surface-enhanced Raman scattering (SERS) to immunology. In the proposed system, antibody immobilized on a solid substrate reacts with antigen, which binds with another antibody labeled with peroxidase. If this immunocomplex is subjected to reaction with o-phenylenediamine and hydrogenperoxide at 37°C, azoaniline is generated. This azo compound is adsorbed on a silver colloid and only the azo compound gives a strong surface-enhanced resonance Raman (SERRS) spectrum. A linear relationship was observed between the peak intensity of the N=N stretching band and the concentration of antigen, revealing that one can determine the concentration of antigen by the SERRS measurement of the reaction product.

Citation Download Citation

Haiying Zhao and Xiaoming Dou "Enzyme immunoassay by dynamic enhanced vibrational spectroscopy of the enzyme reaction product", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.575874

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