A novel method for determination of aflatoxin B1 mediated by FCLA + BSA

WenLi Chen; Da Xing

doi:10.1117/12.572821

14 February 2005 A novel method for determination of aflatoxin B1 mediated by FCLA + BSA

WenLi Chen, Da Xing

Proceedings Volume 5634, Advanced Sensor Systems and Applications II; (2005) https://doi.org/10.1117/12.572821
Event: Photonics Asia, 2004, Beijing, China

Abstract

As a chemiluminescence (CL) probe, 3,7-dihydro-6-{4-{2-(N’-(5-fluoresceinyl) thioureido)ethoxy}phenyl}-2-met -hylimi-dazo{1,2-a}pyrazin-3-one dosium salt (FCLA) can sensitively and specifically react with singlet oxygen (¹O₂ ) and superoxide(O₂""). BSA (Bovine Serum Albumin) can enlarge the CL intensity of FCLA to 860%. This report presents a novel method for determination of Aflatoxin B1 (AfB1) mediated by FCLA+BSA. The concentration of AFB1 showed an obvious positive correlation with the CL intensity mediated by FCLA+BSA. This method could measure accurately ng/ml of AfB1 concentration. At the same time, the fluorescence spectrum of FCLA+BSA and FCLA+BSA+AfB1 were measured respectively, which showed that the fluorescence intensity of FCLA+BSA+AfB1 was higher than FCLA+BSA. Comparing the peak value of FCLA, FCLA+BSA and FCLA+BSA+AfB1 had a 6nm Einstein shift (red shift). The study suggested that CL method mediated by FCLA+BSA might be applicable to the determination of AfB1 concentration.

Citation Download Citation

WenLi Chen and Da Xing "A novel method for determination of aflatoxin B1 mediated by FCLA + BSA", Proc. SPIE 5634, Advanced Sensor Systems and Applications II, (14 February 2005); https://doi.org/10.1117/12.572821

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