Photoacoustic detection of neovascularities in skin graft

Mutsuo Yamazaki; Shunichi Sato; Daizo Saitoh; Miya Ishihara; Yoshiaki Okada; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.588879

25 April 2005 Photoacoustic detection of neovascularities in skin graft

Mutsuo Yamazaki, Shunichi Sato, Daizo Saitoh, Miya Ishihara, Yoshiaki Okada, Hiroshi Ashida, Minoru Obara

Proceedings Volume 5686, Photonic Therapeutics and Diagnostics; (2005) https://doi.org/10.1117/12.588879
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We previously proposed a new method for monitoring adhesion of skin graft by measuring photoacoustic (PA) signal originated from the neovascularities. In this study, immunohistochemical staining (IHC) with CD31 antibody was performed for grafted skin tissue to observe neovascularity, and the results were compared with PA signals. We also used a laser Doppler imaging (LDI) to observe blood flow in the grafted skin, and sensitivity of PA measurement and that of LDI were compared. In rat autograft models, PA signals were measured for the grafted skin at postgrafting times of 0-48 h. At 6 h postgrafting, PA signal was observed in the skin depth region of 500-600 mm, while the results of IHC showed that angiogenesis occurred at the depth of about 600 mm. Depths at which PA signal and angiogenesis were observed decreased with postgrafting time. These indicate that the PA signal observed at 6 h postgrafting originated from the neovascularities in the skin graft. Results of LDI showed no blood-originated signal before 48 h postgrafting. These findings suggest that PA measurement is effective in monitoring the adhesion of skin graft in early stage after transplantation.

Citation Download Citation

Mutsuo Yamazaki, Shunichi Sato, Daizo Saitoh, Miya Ishihara, Yoshiaki Okada, Hiroshi Ashida, and Minoru Obara "Photoacoustic detection of neovascularities in skin graft", Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); https://doi.org/10.1117/12.588879

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