Automated insulin granule segmentation from electron photomicrographs of rat pancreatic ß-cells

Timothy P. McClanahan; Susanne G. Straub; Geoffrey W. G. Sharp; Murray Loew

doi:10.1117/12.595637

14 April 2005 Automated insulin granule segmentation from electron photomicrographs of rat pancreatic β-cells

Timothy P. McClanahan, Susanne G. Straub, Geoffrey W. G. Sharp, Murray Loew

Proceedings Volume 5746, Medical Imaging 2005: Physiology, Function, and Structure from Medical Images; (2005) https://doi.org/10.1117/12.595637
Event: Medical Imaging, 2005, San Diego, California, United States

Abstract

Increased blood glucose stimulates pancreatic β-cells and induces an exocytotic release of insulin. The β-cell, which contains ~10^{^4}insulin-containing granules, releases only a few percent of the granules during a given stimulus such as a meal. The temporal response function to a square wave increase in the concentration of glucose is characteristically biphasic. It is not known whether the granules exhibit random or directed migration patterns as a function of phase. Directed migration would suggest the development of an intracellular gradient directing the path and velocity of insulin granule movement. Our ongoing research investigates this process using manual morphometric analysis of electron micrographs of rat pancreatic β-cells. This is a tedious and time-consuming stereological process. Consequently, we have developed an automated algorithm for accurately segmenting and deriving granule counts, areas, and measuring distance to the plasma membrane. The method is a data-driven image processing approach that implements Mahalanobis classifiers to hierarchically classify pixel candidates and subsequently pixel aggregates as insulin granules. Granule cores and halos are classified independently and fused by intersecting the convex difference of granule halos with core candidates. Once fused, total and individual granule areas and distance metrics to the β-cell plasma membrane are obtained. This algorithm provides a rapid and accurate method for the determination of granule numbers, location, and potential gradients in the pancreatic β-cell under different experimental conditions.

Citation Download Citation

Timothy P. McClanahan, Susanne G. Straub, Geoffrey W. G. Sharp, and Murray Loew "Automated insulin granule segmentation from electron photomicrographs of rat pancreatic β-cells", Proc. SPIE 5746, Medical Imaging 2005: Physiology, Function, and Structure from Medical Images, (14 April 2005); https://doi.org/10.1117/12.595637

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KEYWORDS

Image processing

Image segmentation

Plasma

Photomicroscopy

Algorithm development

Glucose

Mahalanobis distance

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