PCR detection using nanofabricated SERS microchips

N. Elejalde; H. M. H. Chong; O. Inya-Agha; J. M. Cooper

doi:10.1117/12.599857

16 May 2005 PCR detection using nanofabricated SERS microchips

N. Elejalde, H. M. H. Chong, O. Inya-Agha, J. M. Cooper

Proceedings Volume 5763, Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology; (2005) https://doi.org/10.1117/12.599857
Event: SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, 2005, San Diego, California, United States

Abstract

This paper describes a novel method for the fabrication of nanodot arrays with 30nm period which will be used as a platform for the highly sensitive and specific Surface enhanced Raman spectroscopy (SERS) detection of the polymerase chain reaction (PCR). The usual detection methods for PCR involve time consuming methods of DNA labelling, using labels that are capable of altering original DNA properties. We present a detection method which has the advantages of being label free, requiring small analyte volumes and achieving high sensitivity due to SERS enhancement. The required reproducible SERS environment is achieved by the nanofabrication of gold pillars on glass with the use of an electron-beam writer.

Citation Download Citation

N. Elejalde, H. M. H. Chong, O. Inya-Agha, and J. M. Cooper "PCR detection using nanofabricated SERS microchips", Proc. SPIE 5763, Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology, (16 May 2005); https://doi.org/10.1117/12.599857

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