Multiphoton imaging of an in vitro ovarian tissue model

Nathaniel D. Kirkpatrick; Molly A Brewer; Urs Utzinger

doi:10.1117/12.632938

7 October 2005 Multiphoton imaging of an in vitro ovarian tissue model

Nathaniel D. Kirkpatrick, Molly A Brewer, Urs Utzinger

Author Affiliations +

Proceedings Volume 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II; 586002 (2005) https://doi.org/10.1117/12.632938
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

In order to understand the distribution of endogenous fluorescence in the ovary, ovarian biopsies were maintained with a viable tissue imaging system and characterized with multiphoton imaging. It was imperative to maintain a stable in vitro environment so that tissue images could provide accurate correlative data for in vivo spectroscopic measurements. Evaluating tissue viability in real time poses a difficult task given that viability assays are tailored for cell culture. The focus of this study was to design a robust in vitro imaging chamber for assessment of ovarian autofluorescence and simple, reliable viability assays for tissue status monitoring.

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Nathaniel D. Kirkpatrick, Molly A Brewer, and Urs Utzinger "Multiphoton imaging of an in vitro ovarian tissue model", Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 586002 (7 October 2005); https://doi.org/10.1117/12.632938

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