Digital holographic microscopy for study cellular dynamics

Pierre Marquet; Benjamin Rappaz; Etienne Cuche; Yves Emery; Christian D. Depeursinge; Pierre Magistretti

doi:10.1117/12.632917

1 September 2005 Digital holographic microscopy for study cellular dynamics

Pierre Marquet, Benjamin Rappaz, Etienne Cuche, Yves Emery, Christian D. Depeursinge, Pierre Magistretti

Proceedings Volume 5864, Novel Optical Instrumentation for Biomedical Applications II; 58640C (2005) https://doi.org/10.1117/12.632917
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DHM images of living cells in culture are presented. They represent the distribution of the optical path length over the cell, which contains information concerning both the cellular morphometry and the intracellular refractive index, and which has been measured with a sub-wavelength accuracy.

Citation Download Citation

Pierre Marquet, Benjamin Rappaz, Etienne Cuche, Yves Emery, Christian D. Depeursinge, and Pierre Magistretti "Digital holographic microscopy for study cellular dynamics", Proc. SPIE 5864, Novel Optical Instrumentation for Biomedical Applications II, 58640C (1 September 2005); https://doi.org/10.1117/12.632917

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