An all-glass microfluidic cell for the ABEL trap: fabrication and modeling

Adam E. Cohen; W. E. Moerner

doi:10.1117/12.622418

26 August 2005 An all-glass microfluidic cell for the ABEL trap: fabrication and modeling

Adam E. Cohen, W. E. Moerner

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 59300S (2005) https://doi.org/10.1117/12.622418
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

The Anti-Brownian Electrophoretic trap (ABEL trap) allows a user to trap and manipulate individual fluorescent molecules in solution. The heart of the ABEL trap is a microfluidic cell. In previous incarnations of the ABEL trap, the microfluidic cell was formed from a polydimethylsiloxane (PDMS) stamp and a glass coverslip. Here we present an improved microfluidic cell, made entirely out of glass. This new design significantly decreases the rate of photobleaching, which previously limited the time that a single molecule could be trapped. Chemical modifications to the surface of the cell prevent adsorption and allow one to control the balance between electroosmotic and electrophoretic forces. The depth of the trapping region in the cell can be adjusted to allow trapping of different-sized objects.

Citation Download Citation

Adam E. Cohen and W. E. Moerner "An all-glass microfluidic cell for the ABEL trap: fabrication and modeling", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59300S (26 August 2005); https://doi.org/10.1117/12.622418

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