Variation of entropic elasticity of DNA-Psoralen complex under UV light

M. S. Rocha; O. N. Mesquita

doi:10.1117/12.619128

26 August 2005 Variation of entropic elasticity of DNA-Psoralen complex under UV light

M. S. Rocha, O. N. Mesquita

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 593011 (2005) https://doi.org/10.1117/12.619128
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

We measure the entropic elasticity of a single λ-DNA molecule and of a single DNA-Psoralen complex by doing stretching experiments with an optical tweezers. Psoralen is a photosensitive drug used in the treatment of many skin diseases, by impeding DNA replication. Psolaren intercalates the DNA and can form crosslinks with pyrimidine basis in opposite strands of DNA, when illuminated with UVA light. As crosslinks form the persistence length of the complex increases, indicating an increase in rigidity of the complex. We study the kinetics of DNA-Psoralen crosslink formation via changes in entropic elasticity of the complex.

Citation Download Citation

M. S. Rocha and O. N. Mesquita "Variation of entropic elasticity of DNA-Psoralen complex under UV light", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593011 (26 August 2005); https://doi.org/10.1117/12.619128

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available