Luminescence investigation of endothelial cells using metallic and semiconductor nanocrystals

Elena Bogdanovic; Ludmila Bakueva; Lukasz Brzozowski; Ivan Gorelikov; Nikita Reznik; Daniel J. Dumont; J. A. Rowlands

doi:10.1117/12.628234

12 October 2005 Luminescence investigation of endothelial cells using metallic and semiconductor nanocrystals

Elena Bogdanovic, Ludmila Bakueva, Lukasz Brzozowski, Ivan Gorelikov, Nikita Reznik, Daniel J. Dumont, J. A. Rowlands

Proceedings Volume 5969, Photonic Applications in Biosensing and Imaging; 596906 (2005) https://doi.org/10.1117/12.628234
Event: Photonics North, 2005, Toronto, Canada

Abstract

Our aim is to use nanocrystals (NC) to study endothelial cell biology, in particular, the cell surface receptor Tie2. Tie2 is highly expressed in endothelial cells and is critical for angiogenesis and vascular maintenance. Conjugating NCs to the Tie2 ligands, the angiopoietins, and tracking their characteristic luminescence lines will allow us to study the regulation of Tie2 in vitro and in vivo. To study NC behavior in a relevant biological system, endothelial cells were grown and cultured in vitro. Two different types of NC were made and tested: two-color core-shell CdSe/ZnS and more complicated nanostructures containing also Au clusters. Measurements were performed in specially prepared media with different pH values, as well as in the cell growth medium. The positions of NC-related luminescence lines were not influenced by the kind of media which makes them suitable for biolabeling the cell surface receptor Tie2. At the same time, the relative magnitude of the NC peaks depends on the pH of the medium and can therefore be used for characterization of the latter. Confocal microscopic images show that NCs with different ligands demonstrate different distributions inside living cells.

Citation Download Citation

Elena Bogdanovic, Ludmila Bakueva, Lukasz Brzozowski, Ivan Gorelikov, Nikita Reznik, Daniel J. Dumont, and J. A. Rowlands "Luminescence investigation of endothelial cells using metallic and semiconductor nanocrystals", Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 596906 (12 October 2005); https://doi.org/10.1117/12.628234

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Nanocrystals

Luminescence

Gold

Receptors

Confocal microscopy

Semiconductors

In vitro testing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years