Study on the combination of confocal scanning microscopy and defocusing imaging

Zenghui Zhou; Li Liu; Qin Yu; Guiying Wang; Zhizhan Xu

doi:10.1117/12.667171

2 February 2006 Study on the combination of confocal scanning microscopy and defocusing imaging

Zenghui Zhou, Li Liu, Qin Yu, Guiying Wang, Zhizhan Xu

Proceedings Volume 6026, ICO20: Biomedical Optics; 60260R (2006) https://doi.org/10.1117/12.667171
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

The combination of the confocal scanning microscopy and the defocusing imaging in the plane of imaging system was proposed. A switch lens and a pinhole with fine focusing aid are used to switch between the confocal scanning imaging mode and defocusing imaging mode. Objectives of different magnification can be used to get different field of vision. The complementary images can offer more information than any single one. Image blur or distortion caused by scanning, such as the displacement of the target and the excursion of scanning stage, can be corrected according to the comparison of two images. Location and adjustment of the target can be achieved easily and the accuracy of scanning image will be improved obviously. Some samples were tested on a home-built system.

Citation Download Citation

Zenghui Zhou, Li Liu, Qin Yu, Guiying Wang, and Zhizhan Xu "Study on the combination of confocal scanning microscopy and defocusing imaging", Proc. SPIE 6026, ICO20: Biomedical Optics, 60260R (2 February 2006); https://doi.org/10.1117/12.667171

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available