Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics

S. T. McCain; M. E. Gehm; Y. Wang; N. P. Pitsianis; D. J. Brady

doi:10.1117/12.646755

27 February 2006 Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics

S. T. McCain, M. E. Gehm, Y. Wang, N. P. Pitsianis, D. J. Brady

Proceedings Volume 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry; 60930P (2006) https://doi.org/10.1117/12.646755
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have designed and constructed a multimodal multiplex Raman spectrometer which uses multi-wavelength excitation to better detect signals in the presence of fluorescence by taking advantage of the shift-variance of the Raman signal with respect to excitation frequency. Coupled with partial-least-squares (PLS) regression, the technique applied to ethanol estimation in a tissue phantom achieves root-mean-squared-cross-validation errors (RMSCVE) of 9.2 mmol/L with a model formed with 2 principal components, compared to a single wavelength data set with equivalent energy where 7 principal components were used to achieve an RMSCVE of 39.1 mmol/L.

Citation Download Citation

S. T. McCain, M. E. Gehm, Y. Wang, N. P. Pitsianis, and D. J. Brady "Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics", Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930P (27 February 2006); https://doi.org/10.1117/12.646755

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