Digital inline holography of biological specimens

James P. Ryle; Unnikrishnan Gopinathan; Susan McDonnell; Thomas J. Naughton; John T. Sheridan

doi:10.1117/12.680798

30 August 2006 Digital inline holography of biological specimens

James P. Ryle, Unnikrishnan Gopinathan, Susan McDonnell, Thomas J. Naughton, John T. Sheridan

Proceedings Volume 6311, Optical Information Systems IV; 63110C (2006) https://doi.org/10.1117/12.680798
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Digital Holography is the technique of numerically reconstructing a three-dimensional (3D) image containing both amplitude and phase information from a two dimensional (2D) interference pattern recorded by the CCD. In this paper, we study the effects of varying the coherence length by using light from two types of source's (1) coherent laser light and (2) spatially filtered incoherent light from a Light Emitting Diode (LED). We present results using both calibrated test objects and biological samples with view to developing a 3D object recognition and classification system.

Citation Download Citation

James P. Ryle, Unnikrishnan Gopinathan, Susan McDonnell, Thomas J. Naughton, and John T. Sheridan "Digital inline holography of biological specimens", Proc. SPIE 6311, Optical Information Systems IV, 63110C (30 August 2006); https://doi.org/10.1117/12.680798

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