Genome size estimation: a new methodology

Josué Álvarez-Borrego; Cristian Gallardo-Escárate; Vitaly Kober; Oscar López-Bonilla

doi:10.1117/12.742292

16 May 2007 Genome size estimation: a new methodology

Josué Álvarez-Borrego, Cristian Gallardo-Escárate, Vitaly Kober, Oscar López-Bonilla

Proceedings Volume 6422, Sixth Symposium Optics in Industry; 642204 (2007) https://doi.org/10.1117/12.742292
Event: Sixth Symposium Optics in Industry, 2007, Monterrey, Mexico

Abstract

Recently, within the cytogenetic analysis, the evolutionary relations implied in the content of nuclear DNA in plants and animals have received a great attention. The first detailed measurements of the nuclear DNA content were made in the early 40's, several years before Watson and Crick proposed the molecular structure of the DNA. In the following years Hewson Swift developed the concept of "C-value" in reference to the haploid phase of DNA in plants. Later Mirsky and Ris carried out the first systematic study of genomic size in animals, including representatives of the five super classes of vertebrates as well as of some invertebrates. From these preliminary results it became evident that the DNA content varies enormously between the species and that this variation does not bear relation to the intuitive notion from the complexity of the organism. Later, this observation was reaffirmed in the following years as the studies increased on genomic size, thus denominating to this characteristic of the organisms like the "Paradox of the C-value". Few years later along with the no-codification discovery of DNA the paradox was solved, nevertheless, numerous questions remain until nowadays unfinished, taking to denominate this type of studies like the "C-value enigma". In this study, we reported a new method for genome size estimation by quantification of fluorescence fading. We measured the fluorescence intensity each 1600 milliseconds in DAPI-stained nuclei. The estimation of the area under the graph (integral fading) during fading period was related with the genome size.

Citation Download Citation

Josué Álvarez-Borrego, Cristian Gallardo-Escárate, Vitaly Kober, and Oscar López-Bonilla "Genome size estimation: a new methodology", Proc. SPIE 6422, Sixth Symposium Optics in Industry, 642204 (16 May 2007); https://doi.org/10.1117/12.742292

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available