Fluorescence of Pc 4 in U87 cells following photodynamic therapy

Davood Varghai M.D.; Kashif Azizuddin; Yusra Ahmad; Nancy L. Oleinick; David Dean

doi:10.1117/12.701338

23 March 2007 Fluorescence of Pc 4 in U87 cells following photodynamic therapy

Davood Varghai M.D., Kashif Azizuddin, Yusra Ahmad, Nancy L. Oleinick, David Dean

Proceedings Volume 6424, Photonic Therapeutics and Diagnostics III; 64242A (2007) https://doi.org/10.1117/12.701338
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Introduction: Given the length of procedures and the brightness of operating room lights, there is concern that photosensitizers used to locate brain tumors and treat them with photodynamic therapy (PDT) may photobleach before they can be fully utilized. The phthalocyanine photosensitizer Pc 4 is resistant to photobleaching. In this study, we tested the hypothesis that exposure of Pc 4-loaded glioma cells to photoactivating light will result in continuing fluorescence of Pc 4. Methods: U87 human glioma cells were cultured in MEM with 5% penicillin/streptomycin, 5% sodium pyruvate, 10% fetal bovine serum, and 25 mM HEPES. These cultures were given 0 or 125 nM Pc 4, followed 2 hours later by three separate exposures of 200 J/cm² of red light (&lgr;_max = 675 nm). Confocal fluorescence images were collected before and after each exposure. Results: Pc 4 fluorescence was localized to cytoplasmic membranes of the U87 glioma cells, as previously seen in other types of cells. After exposure to PDT, Pc 4 fluorescence was not reduced and even increased. Discussion: Pc 4 may be useful for the intra-operative detection of glioma by fluorescence and for PDT, since neither Pc 4 level nor its fluorescence is likely to decrease during exposure to operating room lights.

Citation Download Citation

Davood Varghai M.D., Kashif Azizuddin, Yusra Ahmad, Nancy L. Oleinick, and David Dean "Fluorescence of Pc 4 in U87 cells following photodynamic therapy", Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242A (23 March 2007); https://doi.org/10.1117/12.701338

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