The use of planarians as in vivo animal model to study laser biomodulation effects

Egberto Munin; Neila Maria Rocha Garcia; Allison Gustavo Braz; Sandra Cristina de Souza; Leandro Procópio Alves; Miguel Angel Castillo Salgado; Viviane Pilla

doi:10.1117/12.701304

19 February 2007 The use of planarians as in vivo animal model to study laser biomodulation effects

Egberto Munin, Neila Maria Rocha Garcia, Allison Gustavo Braz, Sandra Cristina de Souza, Leandro Procópio Alves, Miguel Angel Castillo Salgado, Viviane Pilla

Author Affiliations +

Proceedings Volume 6435, Optical Interactions with Tissue and Cells XVIII; 64350T (2007) https://doi.org/10.1117/12.701304
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A variety of effects is attributed to the photo stimulation of tissues, such as improved healing of ulcers, analgesic and anti-inflammatory effects, stimulation of the proliferation of cells of different origins and stimulation of bone repair. Some investigations that make qualitative evaluations, like wound healing and evaluation of pain and edema, can be conducted in human subjects. However, deeper investigations on the mechanisms of action of the light stimulus and other quantitative works that requires biopsies or destructive analysis has to be carried out in animal models or in cell cultures. In this work, we propose the use of planarians as a model to study laser-tissue interaction. Contrasting with cell cultures and unicellular organisms, planarians are among the simplest organism having tissue layers, central nerve system, digestive and excretory system that might have been platforms for the evolution of the complex and highly organized tissues and organs found in higher organisms. For the present study, 685 nm laser radiation was employed. Planarians were cut transversally, in a plane posterior to the auricles. The body fragments were left to regenerate and the proliferation dynamics of stem cells was studied by using histological analysis. Maximum cell count was obtained for the laser treated group at the 4^th experimental day. At that experimental time, we also had the largest difference between the irradiated and the non-irradiated control group. We concluded that the studied flatworm could be an interesting animal model for in vivo studies of laser-tissue interactions.

Citation Download Citation

Egberto Munin, Neila Maria Rocha Garcia, Allison Gustavo Braz, Sandra Cristina de Souza, Leandro Procópio Alves, Miguel Angel Castillo Salgado, and Viviane Pilla "The use of planarians as in vivo animal model to study laser biomodulation effects", Proc. SPIE 6435, Optical Interactions with Tissue and Cells XVIII, 64350T (19 February 2007); https://doi.org/10.1117/12.701304

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Animal model studies

Organisms

Tissues

In vivo imaging

Stem cells

Laser tissue interaction

Biological research

Show All Keywords

Keywords/Phrases

Search In:

Publication Years